Method validation is a critical aspect of method development in ISHI-Veg. Performance criteria, such as analytical specificity, analytical sensitivity, selectivity, repeatability and reproducibility are measured to demonstrate that the method is fit for purpose. The methods follow ISHI-Veg best practices for PCR, ELISA and dilution-plating assays used in routine seed health testing. The best practice documents identify the number and types of controls necessary for a robust seed health test.
ISHI-Veg methods are reviewed at regular intervals and revised, if found necessary. Therefore, it is the responsibility of the user to check that the most recent version of the protocol is being applied.
For more information on the principles underlying ISHI-Veg methods and best practices for different assays used in ISHI-Veg’s methods, see https://www.worldseed.org/our-work/phytosanitary-matters/seed-health/ishi-veg-method-development/.
ISF cannot guarantee that labs following these protocols will obtain similar results. Many factors, such as staff skills, lab equipment and conditions, reagents and sampling methods can influence the results. Consequently, in case of any litigation ISF will not accept any liability on the use of these tests.
Xanthomonas campestris pv. campestris and X.c. pv. raphani (untreated seed)
Xanthomonas campestris pv. campestris (disinfested/disinfected seed)
Alternaria dauci (Blotter/agar)
Alternaria radicina (Blotter/agar)
A. radicina (A. radicina selective agar)